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SLU-PP-332 features a compact aromatic scaffold with a pyrrole-indole core substituted by phenyl and amide groups, conferring its selectivity for ERR binding; its molecular formula is C18H14N2O2, with a molecular weight of 290.3 g/mol, making it highly amenable to cellular penetration and assay integration. This structure supports solubility in organic solvents like DMSO (up to 100 mg/mL) and moderate aqueous solubility when formulated appropriately, while remaining stable under standard lab conditions away from extreme pH or light exposure. Our preparations achieve purity levels exceeding 98% by HPLC, with consistent batch-to-batch reproducibility that we document transparently, so you can focus on your hypotheses with reliable materials at hand.
SLU-PP-332 is a synthetic small-molecule agonist of the estrogen-related receptors (ERRs), particularly potent at ERRα, designed to activate transcriptional programs that mimic the metabolic adaptations of aerobic exercise in cellular models. By engaging ERRα, β, and γ isoforms, it promotes mitochondrial biogenesis, enhances fatty acid oxidation, and boosts energy expenditure, positioning it as a valuable compound for in-vitro studies exploring metabolic regulation, oxidative metabolism, and cellular resilience to stressors like nutrient excess or aging. Remember, our SLU-PP-332 is supplied strictly for research purposes and in-vitro use only—not for human consumption or any diagnostic or therapeutic applications.
In mechanistic terms, SLU-PP-332 binds to ERRs with high affinity (EC50 of 98 nM for ERRα), inducing a gene expression profile akin to endurance training, upregulating pathways involved in lipid metabolism, the Krebs cycle, and oxidative phosphorylation. This activation shifts cellular fuel preference toward fats, reducing reliance on glucose and mitigating lipid accumulation in models of metabolic dysfunction. In vitro, it elevates expression of PGC-1α coactivators and mitochondrial markers like OXPHOS subunits, fostering a more efficient energy-producing state without the confounding variables of physical activity—we're happy to walk through how this translates to your specific cell lines, offering straightforward interpretations grounded in the data.
In summary, SLU-PP-332 emerges as a precise tool for dissecting ERR-mediated metabolic pathways, enabling researchers to simulate exercise-like benefits in vitro and illuminate strategies for combating metabolic disorders with clarity and control. Its role in enhancing cellular energy dynamics underscores our quiet enthusiasm for compounds that bridge basic science and translational potential, all while upholding the highest standards of quality. Strictly for in-vitro research, SLU-PP-332 reflects our humble dedication to empowering your discoveries through accessible, evidence-driven support. Let's chat about integrating it into your next experiment, one measured insight at a time.
SLU-PP-332's ERR agonism makes it ideal for in-vitro investigations into metabolic remodeling, such as in hepatocyte cultures where it attenuates lipid droplet formation and enhances β-oxidation to model interventions against hepatic steatosis. In adipocyte models, treatment reduces triglyceride storage and promotes browning-like phenotypes, providing insights into obesity-related energy imbalance. Skeletal muscle cell lines benefit from its induction of mitochondrial density and capillary-like networks, useful for studying sarcopenia or endurance adaptations in aging contexts. Additionally, in pancreatic β-cell assays, it supports glucose-stimulated insulin secretion by optimizing mitochondrial function, aiding explorations of diabetes mechanisms. We appreciate the nuance in these applications and are here to suggest dosing ranges or controls that align with your experimental design, keeping things logical and approachable.
For optimal performance in your in-vitro workflows, store SLU-PP-332 as a lyophilized powder at -20°C in a desiccated, amber vial to shield it from moisture and photodegradation. Reconstitute in DMSO or a compatible vehicle like 10% Tween-20 in PBS to achieve stock concentrations of 10-50 mM, then dilute into media for final working levels of 1-10 μM, ensuring even dispersion without precipitation. Aliquot and freeze stocks to limit freeze-thaw cycles, which could impact potency; short-term solutions at 4°C are viable for days but monitor for any solvent evaporation. Backed by our stability validations, these steps help maintain integrity—reach out if you'd like tailored tweaks based on your setup.
